PPT Slide
Development of resistance mutations: in vitro study
Study of appearence of mutations under drug pressure: selection of resistant strains
Evaluation of viral replication in vitro in presence of drugs: determination of IC50 values
Strong CPE on MT-2 cells with ROD strain
MT-4 cell line/MTT assay: quantitative evaluation of CPE
Quantitative real-time PCR
If IC50 value increase, a phenotype of resistance is observed
Sequencing of appropriate region of the viral genome
Comparaison with results obtained in patients
Correlation between phenotype and genotype